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  • 产品名称：6-phosphogluconolactonase(PGLS)(AA1-258)protein(Histag)

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  • 产品厂商：ATGen
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简单介绍：
6-phosphogluconolactonase(PGLS)(AA1-258)protein(Histag)
详情介绍：
Characteristics PGLS, 1-258aa, Human, His tag, E.coli
Purity > 95 % by SDS - PAGE
Background 6-phosphogluconolactonase, also known PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate. Recombinant human PGLS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: 6-phosphogluconolactonase, 6PGL. NCBI no.: NP_036220
Molecular Weight 29.7 kDa (278aa) confirmed by MALDI-TOF
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml (determined by Bradford assay)
Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Storage 4 °C
Supplier Images
SDS-PAGE (SDS) image for 6-phosphogluconolactonase (PGLS) (AA 1-258) protein (His tag) (ABIN667246) 6-phosphogluconolactonase (PGLS) (AA 1-258) protein (His tag)
Background publications Hong, Kim, Kim, Jung, Ahn, Kim, Lee, Kang: "Expression, crystallization and preliminary X-ray crystallographic analysis of Xoo2316, a predicted 6-phosphogluconolactonase, from Xanthomonas oryzae pv. oryzae." in: Acta crystallographica. Section F, Structural biology and crystallization communications, Vol. 64, Issue Pt 11, pp. 1016-8, 2008 (PubMed).

Collard, Collet, Gerin, Veiga-da-Cunha, Van Schaftingen: "Identification of the cDNA encoding human 6-phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway(1)." in: FEBS letters, Vol. 459, Issue 2, pp. 223-6, 1999 (PubMed).