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  • 产品名称：ProSAP-InteractingProtein1(PROSAPIP1)(AA1-673)protein(Histag)

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  • 产品厂商：BioLeaf
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简单介绍：
ProSAP-InteractingProtein1(PROSAPIP1)(AA1-673)protein(Histag)
详情介绍：
Sequence MAKLETLPVR ADPGRDPLLA FAPRPSELGP PDPRLAMGSV GSGVAHAQEF AMKSVGTRTG GGGSQGSFPG PRGSGSGASR ERPGRYPSED KGLANSLYLN GELRGSDHTD VCGNVVGSSG GSSSSGGSDK APPQYREPSH PPKLLATSGK LDQCSEPLVR PSAFKPVVPK NFHSMQNLCP PQTNGTPEGR QGPGGLKGGL DKSRTMTPAG GSGSGLSDSG RNSLTSLPTY SSSYSQHLAP LSASTSHINR IGTASYGSGS GGSSGGGSGY QDLGTSDSGR ASSKSGSSSS MGRPGHLGSG EGGGGGLPFA ACSPPSPSAL IQELEERLWE KEQEVAALRR SLEQSEAAVA QVLEERQKAW ERELAELRQG CSGKLQQVAR RAQRAQQGLQ LQVLRLQQDK KQLQEEAARL MRQREELEDK VAACQKEQAD FLPRIEETKW EVCQKAGEIS LLKQQLKDSQ ADVSQKLSEI VGLRSQLREG RASLREKEEQ LLSLRDSFSS KQASLELGEG ELPAACLKPA LTPVDPAEPQ DALATCESDE AKMRRQAGVA AAASLVSVDG EAEAGGESGT RALRREVGRL QAELAAERRA RERQGASFAE ERRVWLEEKE KVIEYQKQLQ LSYVEMYQRN QQLERRLRER GAAGGASTPT PQHGEEKKAW TPSRLERIES TEI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human LZTS3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Molecular Weight 72.7 kDa Including tag.
UniProt O60299
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Comment

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
image for ProSAP-Interacting Protein 1 (PROSAPIP1) (AA 1-673) protein (His tag) (ABIN3083365) „Crystallography Grade“ protein due to multi-step, protein-specific purification process