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  • 产品名称：Goatanti-RabbitIgG(HeavyChain),(Chaingamma)Antibody(FcTag)

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  • 产品厂商：ArborAssays
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简单介绍：
Goatanti-RabbitIgG(HeavyChain),(Chaingamma)Antibody(FcTag)
详情介绍：
Brand AbX™
Immunogen Highly purified Rabbit IgG Fc portion
Isotype IgG
Cross-Reactivity (Details) Based upon IEP, no reactivity is seen to non-immunoglobulin serum proteins, light chains on all rabbit immunoglobulins, or rabbit F(ab)2 fragments.
Characteristics Coating antibody for enzyme immunoassays, Specific for Fc portion of Rabbit IgG
Purification Affinity purified against Rabbit IgG, Fc portion.
Purity high
Background Solid phase immunoassay utilizing microtiter plates are the predominant assay format for the detection of low concentration small molecules in biological samples. The primary antibody to these small molecules serves two purposes, it confers specificity for the assay by selectively binding the small molecule, and, in binding it deter- mines the sensitivity of the assay. Small molecule assays require a competitive immunoassay technique where the small molecule antigen binds to a limiting amount of the primary antibody in competition with a labeled re- porter small molecule, such as HRP. Primary antibodies can be coated on solid phases but the technique is difficult to reproducibly accomplish, as the dilution of the primary antibody has to be tightly controlled across all of the coated plates and the amounts of pri- mary antibody required can be high. Secondary generic coated plates using goat anti-rabbit IgG antibody coat- ing can be carried out easily and after being blocked and dried these plates are stable for many years. Typical primary antibody titers using coated generic plates can be 10 fold lower than directly coated primary antibodies.
Research Area Immunology, Secondary Antibodies
Application Notes As a plate coating antibody for assays that utilize rabbit polyclonal antibodies as the primary antibody. suGGesteD use: 10 μg/mL in diluted Arbor Assays Coating Buffer
Comment

Affinity Purified Antibody to Rabbit IgG, Fc Extend primary antibody supplies Supplied as a PBS solution
Restrictions For Research Use only
Format Liquid
Buffer Affinity purified against Rabbit IgG, Fc portion. In 10 mM sodium phosphate, 0.15M sodium chloride, 0.05 % sodium azide, pH 7.2.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Product cited in: Khonmee, Vorawattanatham, Pinyopummin, Thitaram, Somgird, Punya****withaya, Brown: "Assessment of faecal glucocorticoid metabolite excretion in captive female fishing cats (Prionailurus viverinus) in Thailand." in: Conservation physiology, Vol. 4, Issue 1, pp. cow021, 2016 (PubMed).