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  • 产品名称：Goatanti-HumanIgM(FcRegion)Antibody(TRITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-HumanIgM(FcRegion)Antibody(TRITC)
详情介绍：
Immunogen Purified IgM prepared from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Fragment F(ab')2 fragement
Specificity The antiserum is reacting with to the Fc subunits of IgM.
Characteristics Tetramethylrhodamine isothiocyanate-conjugated F(ab')2 fragments IgG antibodies of polyclonal goat antiserum to human IgM, Fc specific
Fluorochrome/IgG protein molar ratio (F/P) is approximately 2.7.
Purification Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Research Area Secondary Antibodies
Application Notes Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgM. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration 4.7 mg/mL
Buffer PBS, pH 7.2
Preservative Without preservative
Handling Advice Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not ref rozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Storage 4 °C/-20 °C
Storage Comment The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.