Western Blot of Rabbit anti-Swine IgG (H&L) Antibody Lane 1: Swine IgG Load: 100 ng per lane Primary antibody: Swine IgG (H&L) Antibody at 1:1000 for overnight at 4°C Secondary antibody: DyLight™ 649 goat anti-rabbit at 1:20,000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa