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  • 产品名称：Ubiquitin-ConjugatingEnzymeE2N(UBE2N)Peptide

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  • 产品厂商：PromoCell
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简单介绍：
Ubiquitin-ConjugatingEnzymeE2N(UBE2N)Peptide
详情介绍：
Blocked Antibody anti-Ubiquitin Conjugating Enzyme 13 antibody (ABIN1003409)
Characteristics 15 amino acids near the carboxy terminus of human UBC13.
Application Notes UBC13 peptide is used for blocking the activity of the Anti-UBC13 antibody . It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37ºC.
Restrictions For Research Use only
Format Liquid
Concentration 200 µg/mL
Buffer Supplied in PBS pH 7.2 (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. The peptide solution should be gently mixed before use.
Storage -20 °C
Storage Comment Store at -20°C, stable for one year.
Expiry Date 12 months
Product cited in: Sanjay, Fu, Kreibich: "DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 26094-9, 1998 (PubMed).

Kelleher, Gilmore: "DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 4994-9, 1997 (PubMed).

Steller: "Mechanisms and genes of cellular suicide." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1445-9, 1995 (PubMed).

Nakashima, Sekiguchi, Kuraoka, Fukushima, Shibata, Komiyama, Nishimoto: "Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells." in: Molecular and cellular biology, Vol. 13, Issue 10, pp. 6367-74, 1993 (PubMed).