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  • 产品名称：Goatanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(HRP)
详情介绍：
Immunogen Mouse IgG (H&L)
Cross-Reactivity (Details) Based on immunoelectrophoresis, the antibody reacts with the heavy chains on mouse IgG and with light chains common to most mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins, but antibodies may cross-react with immunoglobulins from other species.
Characteristics Goat anti Mouse IgG (H&L), Goat antibody to Mouse Immunoglobulin G (IgG), Heavy & Light chains Horseradish Peroxidase conjugated
Purification Isolated from antisera by Immuno Affinity Chromatography using antigens coupled to agarose beads.
Research Area Immunology, Secondary Antibodies
Application Notes A dilution range of 1:500 - 1:5.000 is suggested for immunohistochemistry on tissue sections, 1:5.000 - 1:100.000 for ELISA and Western blotting with chromogenic substrates and 1:10.000 - 1:200.000 for Western blotting with ECL substrates. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Concentration (detection method): 0.8 mg/mL (prior to lyophilization)
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 1.5 mL distilled water.
Buffer Lyophilized from 0.01 M Sodium Phosphate, 0.21 M Sodium Chloride, pH 7.6 containing 15 mg/mL BSA.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C
Storage Comment Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50 % followed by storage at -28 °C with or without aliquoting.
Expiry Date 12 months