Western Blot of Donkey anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: Goat IgG antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase donkey secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 and 55 kDa for Goat IgG. Other band(s): none.