Western Blot of Goat anti-Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 100 ng per lane. Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG. Other band(s): none.