Western Blot of Peroxidase conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 25 µg per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none.