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  • 产品名称:Rabbitanti-HumanIgEAntibody

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  • 产品厂商:BioLeaf
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简单介绍:
Rabbitanti-HumanIgEAntibody
详情介绍:
Purpose This antibody is designed for the specific localization of human IgE using IHC techniques in formalin-fixed, paraffin- embedded tissue sections. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful to diagnose leukemias, plasmacytomas and kind no-Hodking lymphomas.
Immunogen IgE isolated from a pool of normal human sera.
Isotype IgG
Characteristics Anti-IgA recognizes purified protein IgA.
Purification Concentrated, Protein G chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Staining pattern: Cytoplasmic. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from thyroid or medullary thyroid carcinoma.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for IgE.

Protocol Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Assay Procedure

Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:500 - 1:1000
Incubation:
3. min, RT
Control Tissue: Tonsil

Restrictions For Research Use only
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25 °C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.
Storage 4 °C
Storage Comment Store at 2-8 °C until the expiration date printed on product label.
Supplier Images
Immunohistochemistry (IHC) image for Rabbit anti-Human IgE antibody (ABIN1027286) 10 × 20 × IHC of IgE (Polyclonal) on FFPE tonsil tissue. Citrate pH 6.5, DAB, Hematox...