Western Blot of Alkaline Phosphatase Conjugated Goat anti-Human IgG Gamma Chain antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase human secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa for Human IgG Gamma Chain. Other band(s): none.