Mouseanti-HumanIgG(WholeMolecule)Antibody(FITC) 抗体网

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产品名称：Mouseanti-HumanIgG(WholeMolecule)Antibody(FITC)
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产品厂商：BioLeaf
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简单介绍：

Mouseanti-HumanIgG(WholeMolecule)Antibody(FITC)

详情介绍：

Product details
Product details
Immunogen	Human IgG whole molecule
Isotype	IgG
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
Purification	This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Sterility	Sterile filtered

Target details
Target details
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Optimal working dilutions should be determined experimentally by the investigator.
Comment	Excitation/Emission wavelength: 494 nm/514 nm
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
Concentration	1.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	Sodium azide
Precaution of Use	WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice	Product is photosensitive and should be protected from light.
Storage	4 °C/-20 °C
Storage Comment	Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
Expiry Date	12 months

Images
Images
Supplier Images	Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human Ig... Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescien Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP. Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescien Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.