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  • 产品名称：Rabbitanti-Cow(Bovine)IgG(Heavy&LightChain)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-Cow(Bovine)IgG(Heavy&LightChain)Antibody(HRP)
详情介绍：
Immunogen Bovine IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Bovine IgG antibody detects heavy and light chain of Bovine IgG molecule. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
Characteristics Anti-Bovine IgG is ideal for investigators involved in serum protein components.
Concentration Definition: by UV absorbance at 280 nm
Research Area Immunology, Secondary Antibodies
Application Notes Anti Bovine IgG antibody has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).