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  • 产品名称:Goatanti-Rat(Rattus)IgM(Chainmu)Antibody(AlkalinePhosphatase(AP))

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  • 产品厂商:BioLeaf
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简单介绍:
Goatanti-Rat(Rattus)IgM(Chainmu)Antibody(AlkalinePhosphatase(AP))
详情介绍:
Immunogen Purified rat IgM
Cross-Reactivity (Details) Based on immunoelectrophoresis, the antibody reacts with the heavy chain portion of rat IgM. No antibody was detected against normal rat IgG or non-immunoglobulin serum proteins. However, these antibodies may cross-react with IgM from other species.
Characteristics Goat anti Rat IgM (mu), Goat antibody to Rat Immunoglobulin M (IgM), Mu Chain Specific Alkaline Phosphatase conjugated
Purification The antibody was isolated from antisera by Immuno Affinity Chromatography using antigens coupled to agarose beads.
Research Area Secondary Antibodies
Application Notes A dilution range of 1:5.000 - 1:50.000 is suggested for ELISA and Western blots. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Concentration (detection method): 0.6 mg/mL (prior to lyophilization)

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 mL with distilled water.
Buffer Lyophilized from 0.01 M Tris-HCl, 0.21 M Sodium chloride, pH 8.0 containing 15 mg/mL BSA, 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution only prior to immediate use. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette.
Storage 4 °C
Storage Comment Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50 % glycerol followed by storage at -28 °C with or without aliquoting.
Expiry Date 12 months