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Format
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Lyophilized
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Reconstitution
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Reconstitute with 1 mL distilled water.
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Buffer
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Lyophilized from 0.01 M Sodium Phosphate, 0.21 M Sodium Chloride, pH 7.6 containing 15 mg/mL BSA.
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Preservative
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Sodium azide
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Precaution of Use
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WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
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Storage
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4 °C
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Storage Comment
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Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
To judge clarity, draw product into a pasteur pipette.Prepare working dilution only prior to immediate use. For extended storage after reconstitution, add an equal volume of glycerol (ACS or better grade) for a final glycerol concentration of 50 % followed by storage at -28 °C, with or without aliquotting. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Alternatively, in the absence of glycerol, the product may be aliquot and stored at -78 °C or below.
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Expiry Date
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12 months
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