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  • 产品名称:Goatanti-Mouse(Murine)IgG(FcRegion),(Chaingamma)Antibody(Biotin)

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  • 产品厂商:BioLeaf
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简单介绍:
Goatanti-Mouse(Murine)IgG(FcRegion),(Chaingamma)Antibody(Biotin)
详情介绍:
Immunogen Mouse IgG Fc fragments
Cross-Reactivity (Details) Based on immunoelectrophoresis, the antibody reacts with the heavy chains on mouse IgG but not with the light chains on most mouse immunoglobulins. No antibody was detected against mouse IgM, or against non-immunoglobulin serum proteins, but antibodies may cross react with immunoglobulins from other species.
Characteristics Goat anti Mouse IgG (Fc), Goat antibody to Mouse Immunoglobulin G (IgG), Fc-gamma Fragment Specific Biotin conjugated
Purification Isolated from antisera by Immuno Affinity Chromatography using antigens coupled to agarose beads.
Research Area Immunology, Secondary Antibodies
Application Notes A dilution range of 1:20.000 - 1:400.000 is suggested for ELISA and Western blotting using enzyme-conjugated streptavidin, 1:500 - 1:5.000 for enzyme immunohistochemistry on tissue sections and 1:200 - 1:1.000 for flow cytometry and fluorescence immunohisto/cytochemistry. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Concentration (detection method): 1.2 mg/mL (prior to lyophilization)

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 2 mL distilled water.
Buffer Lyophilized from 0.01 M Sodium phosphate, 0.21 M Sodium chloride, pH 7.6 containing 15 mg/mL BSA, 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Prepare working dilution only prior to immediate use. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a clean pasteur pipette.
Storage 4 °C
Storage Comment Store lyophilized product at 2-8 °C. After reconstitution, product is stable for six weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol (ACS or better grade) to make a final glycerol concentration of 50 % followed by storage at -28 °C with or without aliquoting.
Expiry Date 12 months