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  • 产品名称：Rabbitanti-GoatIgG(FcRegion)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-GoatIgG(FcRegion)Antibody(HRP)
详情介绍：
Immunogen Anti-Goat IgG was produced by repeated immunization with Goat IgG fragment in rabbit.
Isotype IgG
Specificity IgG F(c)
Characteristics Anti-Goat IgG F(c) peroxidase conjugated antibody generated in rabbit detects specifically goat IgG F(c). This secondary peroxidase conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Background Synonyms: Goat IgG F(c) Antibody, Rb-a-Goat IgG F(c) Peroxidase conjugated, goat IgG F(c) Peroxidase Antibody in rabbit, Rabbit peroxidase conjugated Secondary Antibody.
Research Area Immunology, Secondary Antibodies
Application Notes Goat IgG F(c) peroxidase conjugated antibody has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Anti-Goat IgG F(c) peroxidase conjugated antibody has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 2.0 mg/mL
Buffer 0.01 M Potassium Phosphate, 0.14 M Sodium Chloride, pH 7.4
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free Preservative: 0.01 % (w/v) Gentamicin Sulfate
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Ma, Yan, Huang, Zhao, Zhu: "Downregulation of human epidermal growth factor receptor 2 by short hairpin RNA increases chemosensitivity of human ovarian cancer cells." in: Oncology letters, Vol. 9, Issue 5, pp. 2211-2217, 2015 (PubMed).

Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).