产品详情
所在位置: 首页> 产品目录> 二抗>
查看大图

  • 产品名称：Goatanti-MonkeyIgG(FcRegion)Antibody(HRP)

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Goatanti-MonkeyIgG(FcRegion)Antibody(HRP)
详情介绍：
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity.
Characteristics Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to monkey IgG, Fc specific
Peroxidase/IgG protein molar ratio (E/P) is approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
Purification Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Research Area Immunology, Secondary Antibodies
Application Notes In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demon-strate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration 6.5 mg/mL
Buffer Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative Without preservative
Handling Advice Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and pr eferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Storage 4 °C/-20 °C
Storage Comment The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
Product cited in: Sundling, Zhang, Phad, Sheng, Wang, Mascola, Li, Wyatt, Shapiro, Karlsson Hedestam: "Single-cell and deep sequencing of IgG-switched macaque B cells reveal a diverse Ig repertoire following immunization." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 8, pp. 3637-44, 2014 (PubMed).