Western Blot of Rabbit anti-Swine IgG Antibody. Lane 1: Swine IgG. Load: 100 ng per lane. Primary antibody: Swine IgG Antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 goat anti-rabbit at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT.