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  • 产品名称：Mouseanti-HumanIg(Chainlambda),(LightChain)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIg(Chainlambda),(LightChain)Antibody
详情介绍：
Clone 4C2
Isotype IgG1
Specificity The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
No Cross-Reactivity Hamster, Sheep (Ovine), Goat, Rabbit, Guinea Pig
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Molecular Weight 22.5 kDa
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (ABIN457412) B lymphocytes (CD19 +) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain dete...
Background publications Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).