产品详情
查看大图

  • 产品名称：CP43'Peptide

  • 产品型号：
  • 产品厂商：Agrisera
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CP43'Peptide
详情介绍：
Brand SERVA®
Purification affinity purified
Background This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Molecular Weight 27 kDa (slightly larger than native protein due to His-tag)
Application Notes Standard curve: 3 loads are recommended (2.5 and 10 i1/4l).For most applications a sample load of 0.2i1/4g of chlorophyll will give a IsiA signal in this range.Positive control:a 2i1/4l load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Comment

Concentration: after adding 225 μl of milliQ water final concentration of the standard is 0.15 pmoles/μlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 225 μL of milliQ water
Buffer Glycerol 10 %, Tris Base 141 mM, Tris HCl 106 mM, LDS 2 %, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1 mg/mL PefaBloc protease inhibitor (Roche), 50 mM DTT. This standard is ready-to-load and does not require any additions or heating.
Handling Advice Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Storage -20 °C
Storage Comment store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.