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  • 产品名称：Donkeyanti-Sheep(Ovine)IgG(Heavy&LightChain)Antibody(AlkalinePhosphatase(AP))

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  • 产品厂商：Agrisera
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简单介绍：
Donkeyanti-Sheep(Ovine)IgG(Heavy&LightChain)Antibody(AlkalinePhosphatase(AP))
详情介绍：
Isotype IgG
Cross-Reactivity Human, Mouse (Murine), Rabbit
Characteristics Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human, mouse, rabbit IgG and are affinity purified using solid phase sheep IgG.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
Restrictions For Research Use only
Format Liquid
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Storage Comment Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.