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  • 产品名称：Rabbitanti-HumanIgM(Chainmu)Antibody(AlkalinePhosphatase(AP))

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  • 产品厂商：Agrisera
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简单介绍：
Rabbitanti-HumanIgM(Chainmu)Antibody(AlkalinePhosphatase(AP))
详情介绍：
Isotype IgG
Characteristics Rabbit anti-human IgM (µ chain) is an ALP conjugated secondary antibody which binds to human IgM (µ chain) in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Antibody concentration is 1.50 mg/mL (E 1 % at 280 nm = 13.0).
Purification Affinity purification
Purity > 95 % based on SDS-PAGE
Research Area Secondary Antibodies
Application Notes This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2000 for Immunohistochemistry 1:50 000-1:5 000 for ELISA/Western blot.
Comment

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgM Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins
Restrictions For Research Use only
Format Liquid
Buffer 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Storage Comment Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming