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  • 产品名称：Goatanti-HumanIgG(Heavy&LightChain)Antibody(DyLight550)

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  • 产品厂商：Agrisera
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简单介绍：
Goatanti-HumanIgG(Heavy&LightChain)Antibody(DyLight550)
详情介绍：
Immunogen Purified Human IgG, whole molecule
Fragment F(ab')2 fragment
Specificity Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG and and light chains on all human immunoglobulins.
Characteristics Goat anti-human IgG (H&L), F(ab) '2 fragment - DyLight® 550 Conjugate, is a secondary antibody conjugated to DyLight® 550, which binds to all human IgG, F(ab) '2 fragment in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgG.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes 1:20 - 1:2,000 for most applications
Comment

Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on human IgG ( μ chain) light chains on all human immunoglobulins.No reactivity is observed to: non-immunoglobulin human serum proteins serum proteins from bovine, mouse or rabbit.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.