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  • 产品名称：Donkeyanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(DyLight633)

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  • 产品厂商：Agrisera
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简单介绍：
Donkeyanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(DyLight633)
详情介绍：
Immunogen Purified mouse IgG, whole molecule
Specificity Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on mouse IgG and light chains on all mouse immunoglobulins.
Cross-Reactivity Sheep (Ovine), Chicken, Goat, Guinea Pig, Hamster, Horse (Equine), Human, Rabbit, Rat (Rattus)
Characteristics Donkey anti-Mouse IgG (H&L) - DyLight® 633 Conjugated, is a secondary antibody conjugated to DyLight® 633, which binds to mouse IgG (H&L) in immunological assays. DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes 1:20 - 1:2,000 for most applications
Comment

Based on immunoelectrophoresis, this antibody reacts with: (gamma) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to:non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v).
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.