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  • 产品名称：PoliovirusReceptor(PVR)(AA21-343)(Active)protein(FcTag,AVItag,Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
PoliovirusReceptor(PVR)(AA21-343)(Active)protein(FcTag,AVItag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 63.5 kDa. As a result of Glycosylation, the protein migrates as 75-100 kDa under reducing (R) condition, and 140-180 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.
Molecular Weight 63.5 kDa
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Poliovirus Receptor (PVR) (AA 21-343) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN3137677) Biotinylated Human CD155, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) ...
image for Poliovirus Receptor (PVR) (AA 21-343) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN3137677) Measured by its binding ability in a functional ELISA. Immobilized Human DNAM-1, Fc T...
image for Poliovirus Receptor (PVR) (AA 21-343) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN3137677) Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Ta...