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  • 产品名称：ADAMMetallopeptidaseDomain12(ADAM12)(Active)protein(Histag,HeavyLabeled)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ADAMMetallopeptidaseDomain12(ADAM12)(Active)protein(Histag,HeavyLabeled)
详情介绍：
Characteristics ADAM12, Heavy Labeled is fused with 6 xHis tag at the C terminus, and has a calculated MW of 55.2 kDa (Arg 29 - Asp 513, Propeptide 20.1 kDa + mature form 34.5 kDa). The rh ADAM12 (Arg 29 - Asp 513) was cleaved into the propeptide and the mature form. The reducing (R) protein migrates as 65 kDa, 46 kDa and 23 kDa in SDS-PAGE due to glycosylation, corresponding to the proprotein form, the mature form and the propeptide respectively.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background A Disintegrin And Metalloprotease 12 (ADAM12) is also known as Meltrin-alpha and MCMP, is a member of the ADAM and MDC (Metalloprotease, Disintegrin, Cysteine-rich) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE. ADAM12 has four Isoform, Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. ADAM12 Involved in skeletal muscle regeneration, specifically at the onset of cell fusion and also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Molecular Weight 55.7 kDa (Proprotein), 34.8 kDa (Mature Form) and 20.3 kDa (propeptide)
UniProt O43184
Research Area Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Proteases
Pathways EGFR Signaling Pathway
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for ADAM Metallopeptidase Domain 12 (ADAM12) (Active) protein (His tag,Heavy Labeled) (ABIN2444047) ADAM Metallopeptidase Domain 12 (ADAM12) (Active) protein (His tag,Heavy Labeled)