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  • 产品名称：IntegrinalphaVbeta8(AA31-992)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
IntegrinalphaVbeta8(AA31-992)(Active)protein(Histag)
详情介绍：
Characteristics Human ITGAV & ITGB8 Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113 kDa (ITGAV) and 75.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 145 kDa (ITGAV) and 100-116 kDa (ITGB8) respectively due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.
Molecular Weight 113 kDa (ITGAV) & 76.5 kDa (ITGB8)
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM NaCl, 2 mM MgCl2, pH 8.0
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Integrin alpha V beta 8 (AA 31-992) (Active) protein (His tag) (ABIN4949120) Human ITGAV & ITGB8 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The...
image for Integrin alpha V beta 8 (AA 31-992) (Active) protein (His tag) (ABIN4949120) Measured by its binding ability in a functional ELISA. Immobilized Human ITGAVB8, His...