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  • 产品名称：MatrixMetallopeptidase9(GelatinaseB,92kDaGelatinase,92kDaTypeIVCollagenase)(MMP

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  • 产品厂商：ACROBiosystems
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简单介绍：
MatrixMetallopeptidase9(GelatinaseB,92kDaGelatinase,92kDaTypeIVCollagenase)(MMP9)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 50.8 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >1200 pmol/min/μg.
Background Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), CLG4B, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. . Structurally, MMP9 maybe be divided into five distinct domains: a prodomain which is cleaved upon activation, a gelatinbinding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc , a prolinerich linker region, and a carboxyl terminal hemopexinlike domain. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.
Molecular Weight 50.8 kDa
UniProt P14780
Research Area Extracellular Matrix
Pathways Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (Active) protein (His tag) (ABIN2181513) Human MMP-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Mira, Lacalle, Buesa, de Buitrago, Jiménez-Baranda, Gómez-Moutón, Martínez-A, Mañes: "Secreted MMP9 promotes angiogenesis more efficiently than constitutive active MMP9 bound to the tumor cell surface." in: Journal of cell science, Vol. 117, Issue Pt 9, pp. 1847-57, 2004 (PubMed).

Mira, Lacalle, Buesa, de Buitrago, Jiménez-Baranda, Gómez-Moutón, Martínez-A, Mañes: "Secreted MMP9 promotes angiogenesis more efficiently than constitutive active MMP9 bound to the tumor cell surface." in: Journal of cell science, Vol. 117, Issue Pt 9, pp. 1847-57, 2004 (PubMed).