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  • 产品名称：EpidermalGrowthFactorReceptor(EGFR)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
EpidermalGrowthFactorReceptor(EGFR)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 69.5 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized human EGF (Catalog # EGF-H4114) at 2. 5 μg/mL (100 μL/well) can bind rhEGFR (Catalog # EGR-H5222) with a linear range of 0. 1-1 μg/mL. Validated CetuxiMab binding in a functional ELISA.
Background The epidermal growth factor receptor (EGFR, ErbB-1, HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Molecular Weight 69.5 kDa
NCBI Accession NP_005219
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Epidermal Growth Factor Receptor (EGFR) (Active) protein (His tag) (ABIN2180999) Epidermal Growth Factor Receptor (EGFR) (Active) protein (His tag)
Product cited in: Zinn, Korb, Samuel, Warram, Dion, Killingsworth, Fan, Schoeb, Strong, Rosenthal: "IND-directed safety and biodistribution study of intravenously injected cetuximab-IRDye800 in cynomolgus macaques." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 17, Issue 1, pp. 49-57, 2015 (PubMed).

Zinn, Korb, Samuel, Warram, Dion, Killingsworth, Fan, Schoeb, Strong, Rosenthal: "IND-directed safety and biodistribution study of intravenously injected cetuximab-IRDye800 in cynomolgus macaques." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 17, Issue 1, pp. 49-57, 2015 (PubMed).