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  • 产品名称：Transferrin(Tf)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Transferrin(Tf)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76 kDa. The protein migrates as 74 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized Human Transferrin /TF Protein (Cat# TRN-H4229) at 10 μg/mL (100 μL/well), can bind Biotinylated Human CD71 Protein (Cat# TFR-H8243). The EC50 is typically 0. 04. 4 μg/mL.
Background Transferrin is also known as Serotransferrin, Beta-1 metal-binding globulin, TF, and is iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. Although iron bound to transferrin is less than 0.1 % (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). The affinity of transferrin for Fe(III) is extremely high (1023 M-1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.When not bound to iron, it is known as "apo-transferrin". In humans, transferrin consists of a polypeptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains (the first situated in the N-terminus and the second in the C-terminus). The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-. The liver is the main source of manufacturing transferrin, but other sources such as the brain also produce this molecule . Transferrin is also associated with the innate immune system. Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron that impedes bacteria survival in a process called iron withholding. The level of transferrin decreases in inflammation. The metal binding properties of transferrin have a great influence on the biochemistry of plutonium in humans. Transferrin has a bacteriocidal effect on bacteria, in that it makes Fe3+ unavailable to the bacteria.Carbohydrate deficient transferrin increases in the blood with heavy ethanol consumption and can be monitored via laboratory testing.
Molecular Weight 76 kDa
UniProt P02787
Research Area Cardiovascular, Serum/Plasma Proteins, Enzymes, Metabolism
Pathways Transition Metal Ion Homeostasis
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Transferrin (Tf) (Active) protein (His tag) (ABIN2181871) Human Transferrin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Sharpe: "Biochemical detection and monitoring of alcohol abuse and abstinence." in: Annals of clinical biochemistry, Vol. 38, Issue Pt 6, pp. 652-64, 2001 (PubMed).

Sharpe: "Biochemical detection and monitoring of alcohol abuse and abstinence." in: Annals of clinical biochemistry, Vol. 38, Issue Pt 6, pp. 652-64, 2001 (PubMed).

Crichton, Charloteaux-Wauters: "Iron transport and storage." in: European journal of biochemistry / FEBS, Vol. 164, Issue 3, pp. 485-506, 1987 (PubMed).

Crichton, Charloteaux-Wauters: "Iron transport and storage." in: European journal of biochemistry / FEBS, Vol. 164, Issue 3, pp. 485-506, 1987 (PubMed).