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  • 产品名称：LeukemiaInhibitoryFactor(LIF)(AA23-202)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
LeukemiaInhibitoryFactor(LIF)(AA23-202)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics rhLIF, with Gly-Pro at the N- terminus, has a calculated MW of 19.9 kDa. The predicted N-terminus is Ser 23. The reducing (R) protein migrates as 33-45 kDa.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng="" ml,="" corresponding="" to="" a="" specific="" activity="" of="">1X107 Unit/mg.
Background Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Molecular Weight 19.9 kDa
UniProt P15018
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) Protein (ABIN2181462) Human LIF on SDS-PAGE under reducing (R) condition. The gel was stained overnight wit...