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  • 产品名称：FcFragmentofIgG,HighAffinityIa,Receptor(CD64)(FCGR1A)(Active)protein(AVItag,Bio

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  • 产品厂商：ACROBiosystems
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简单介绍：
FcFragmentofIgG,HighAffinityIa,Receptor(CD64)(FCGR1A)(Active)protein(AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 33 kDa. The protein migrates as 45-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
Molecular Weight 33 kDa
NCBI Accession NP_034316
UniProt P12314
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (Active) protein (AVI tag,Biotin,His tag) (ABIN2444068) Biotinylated Mouse Fc gamma RI on SDS-PAGE under reducing (R) condition. The gel was ...
Binding Studies (Bind) image for Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (Active) protein (AVI tag,Biotin,His tag) (ABIN2444068) Immobilized OKT3 mAb at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RI...