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  • 产品名称：ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(Active)protein(AVItag,Biotin,H

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(Active)protein(AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 74.9 kDa. The protein migrates as 20 kDa and 65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Molecular Weight 74.9 kDa
Gene ID 18, 17
UniProt Q80W65
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (Active) protein (AVI tag,Biotin,His tag) (ABIN2870574) Biotinylated Mouse PCSK9 on SDS-PAGE under reducing (R) condition. The gel was staine...
Binding Studies (Bind) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (Active) protein (AVI tag,Biotin,His tag) (ABIN2870574) Immobilized Mouse LDL R Protein, His Tag (Cat# LDR-M52H8 ) at 10 μg/mL (100 μL/well) ...