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  • 产品名称：TCellImmunoreceptorwithIgandITIMDomains(TIGIT)(AA22-141)(Active)protein(FcTag,A

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  • 产品厂商：ACROBiosystems
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简单介绍：
TCellImmunoreceptorwithIgandITIMDomains(TIGIT)(AA22-141)(Active)protein(FcTag,AVItag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 41.5 kDa. As a result of glycosylation, the protein migrates as 47 kDa under reducing (R) condition, and 75-116 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Molecular Weight 41.5 kDa
UniProt Q495A1
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN4949039) Biotinylated Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) ...
Binding Studies (Bind) image for T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN4949039) Immobilized Human CD155, Fc Tag with a linear range of 9.8-78 ng/mL.