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  • 产品名称：VascularEndothelialGrowthFactorA(VEGFA)(AA27-190)(Active)protein(AVItag,Biotin,

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  • 产品厂商：ACROBiosystems
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简单介绍：
VascularEndothelialGrowthFactorA(VEGFA)(AA27-190)(Active)protein(AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 22.5 kDa. As a result of different glycosylation, the protein migrates as 28-33 kDa under reducing (R) condition, and 46-60 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.
Molecular Weight 22.5 kDa
Research Area Signaling, Immunology, Receptors, Growth Factors, Cytokines, Inflammation
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949052) Biotinylated Mouse VEGF164, His Tag on SDS-PAGE under reducing (R) and no-reducing (N...
image for Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949052) Measured by its binding ability in a functional ELISA. Immobilized Human VEGF R1, His...
image for Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949052) Measured by its binding ability in a functional ELISA. Immobilized VEGFR2/R3, Fc tag ...