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  • 产品名称：ProgrammedCellDeath1(PDCD1)(Active)protein(FcTag,Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProgrammedCellDeath1(PDCD1)(Active)protein(FcTag,Biotin)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics This protein carries a human IgG1 Fc fragment at the C-terminus. The protein has a calculated MW of 43.2 kDa. The protein migrates as 60-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized Mouse PD-L1 /B7-H1 Protein Fc chimera(Cat# PD1-M5251) at 5 μg/mL (100 μl/well) can bind Biotinylated Mouse PDCD1 /PD1 Fc Chimera (Cat# PD1-M8259) with a linear range of 0. 2-5 μg/mL .
Background Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
Molecular Weight 43.2 kDa
NCBI Accession NP_032824
UniProt Q02242
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Programmed Cell Death 1 (PDCD1) (Active) protein (Fc Tag,Biotin) (ABIN2444190) Biotinylated Mouse PD-1, Fc tag on SDS-PAGE under reducing (R) condition. The gel was...
Binding Studies (Bind) image for Programmed Cell Death 1 (PDCD1) (Active) protein (Fc Tag,Biotin) (ABIN2444190) Immobilized Mouse PD-L1， Fc Tag (Cat# PD1-M5251) at 5 µg/mL (100 µl/well) can bind Bi...