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  • 产品名称：PlasminogenActivator,Urokinase(PLAU)(AA21-431)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
PlasminogenActivator,Urokinase(PLAU)(AA21-431)(Active)protein(Histag)
详情介绍：
Characteristics Human PLAU, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 45 kDa. The predicted N-terminus is Ser21, Ile179 & Lys156. The reducing (R) protein migrates as four bands corresponding to the short α chain, long α chain, β chain and unprocessed full-length chain with the molecular mass of 20 kDa, 23 kDa, 32 kDa and 50 kDa respectively in SDS-PAGE due to glycosylation and cleavage.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human PLAUR in a function ELISA.

SDS-PAGE: due to glycosylation and cleavage.
Background Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys - Pro - Gly - Arg560 - Val561 - Val - Gly - Gly - Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).
Molecular Weight 45 kDa
NCBI Accession NP_002649
UniProt P00749
Pathways Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Sodium Acetate, 100 mM NaCl, pH 5.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Plasminogen Activator, Urokinase (PLAU) (AA 21-431) (Active) protein (His tag) (ABIN2181654) Human PLAU, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...