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  • 产品名称：KITLigand(KITLG)(Active)protein(StrepTag,Biotin,Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
KITLigand(KITLG)(Active)protein(StrepTag,Biotin,Histag)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-30 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.
Purity >98 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
Background Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.
Molecular Weight 19.1 kDa
NCBI Accession NP_038626
UniProt P20826
Research Area Embryogenesis, Embryonic stem cells, Innate Immunity, Cytokines
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for KIT Ligand (KITLG) (Active) protein (Strep Tag,Biotin,His tag) (ABIN2444217) Biotinylated Mouse SCF on SDS-PAGE under reducing (R) condition. The gel was stained ...
Binding Studies (Bind) image for KIT Ligand (KITLG) (Active) protein (Strep Tag,Biotin,His tag) (ABIN2444217) Immobilized Human CD117, His Tag (Cat# CD7-H52H4) at 2 μg/mL (100 μl/well) can bind B...