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  • 产品名称：Interleukin6(IL6)(Active)protein(Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Interleukin6(IL6)(Active)protein(Biotin)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics The product does NOT contain any epitope tags. The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized Human IL6R /CD126 Protein (Cat# ILR-H4223) at 5μg/mL (100 μL/well), can bind Biotinylated rh IL6 Protein (Cat# IL6-H8218). The EC50 is typically 0. 06. 6 μg/mL.
Background Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.
Molecular Weight 20.8 kDa
NCBI Accession NP_000591
UniProt P05231
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors
Pathways Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Interleukin 6 (IL6) (Active) protein (Biotin) (ABIN2444149) Biotinylated Human IL-6 on SDS-PAGE under reducing (R) condition. The gel was stained...
Binding Studies (Bind) image for Interleukin 6 (IL6) (Active) protein (Biotin) (ABIN2444149) Immobilized Human IL-6 R alpha, His Tag (Cat# ILR-H4223) at 5μg/mL (100 µL/well),can ...