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  • 产品名称：CD3eMolecule,epsilon(CD3-TCRComplex)(CD3E)(Active)protein(Biotin,Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD3eMolecule,epsilon(CD3-TCRComplex)(CD3E)(Active)protein(Biotin,Histag)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.2 kDa. The protein migrates as 18-20 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >90 % as determined by reduced SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
Background CD3e molecule, epsilon also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
Molecular Weight 13.2 kDa
NCBI Accession NP_000724
UniProt P07766
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (Active) protein (Biotin,His tag) (ABIN2444082) Biotinylated Human CD3 epsilon on SDS-PAGE under reducing (R) condition. The gel was ...