产品详情
查看大图

  • 产品名称：ApolipoproteinA-I(APOA1)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
ApolipoproteinA-I(APOA1)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29.8 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Immobilized Human ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
Background ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.
Molecular Weight 29.8 kDa
NCBI Accession NP_000030
UniProt P02647
Pathways Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Apolipoprotein A-I (APOA1) (Active) protein (His tag) (ABIN2180599) Human Apo-A1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Voyiaziakis, Goldberg, Plump, Rubin, Breslow, Huang: "ApoA-I deficiency causes both hypertriglyceridemia and increased atherosclerosis in human apoB transgenic mice." in: Journal of lipid research, Vol. 39, Issue 2, pp. 313-21, 1998 (PubMed).

Voyiaziakis, Goldberg, Plump, Rubin, Breslow, Huang: "ApoA-I deficiency causes both hypertriglyceridemia and increased atherosclerosis in human apoB transgenic mice." in: Journal of lipid research, Vol. 39, Issue 2, pp. 313-21, 1998 (PubMed).

Eggerman, Hoeg, Meng, Tombragel, Bojanovski, Brewer: "Differential tissue-specific expression of human apoA-I and apoA-II." in: Journal of lipid research, Vol. 32, Issue 5, pp. 821-8, 1991 (PubMed).

Eggerman, Hoeg, Meng, Tombragel, Bojanovski, Brewer: "Differential tissue-specific expression of human apoA-I and apoA-II." in: Journal of lipid research, Vol. 32, Issue 5, pp. 821-8, 1991 (PubMed).