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  • 产品名称：Kallikrein11(KLK11)(AA19-250)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Kallikrein11(KLK11)(AA19-250)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is > 300 pmoles / min / μg.
Background Kallikrein 11 (KLK11) is also known as Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK11 contains one peptidase S1 domain. KLK11 is expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). Kallikrein 11 /KLK11 is a possible multifunctional protease and efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine but not lysine residues.
Molecular Weight 26.5 kDa
UniProt Q9UBX7
Pathways Complement System
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Kallikrein 11 (KLK11) (AA 19-250) (Active) protein (His tag) (ABIN2181429) Human Kallikrein 11, His Tag on SDS-PAGE under reducing (R) condition. The gel was st...
Background publications Mitsui, Nakamura, Okui, Kominami, Uemura, Yamaguchi: "Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11/hippostasin." in: The FEBS journal, Vol. 273, Issue 16, pp. 3678-86, 2006 (PubMed).

Luo, Shan, Elliott, Soosaipillai, Diamandis: "Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 3 Pt 1, pp. 742-50, 2006 (PubMed).

Mitsui, Yamada, Okui, Kominami, Uemura, Yamaguchi: "A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate." in: Biochemical and biophysical research communications, Vol. 272, Issue 1, pp. 205-11, 2000 (PubMed).