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  • 产品名称：MembraneMetallo-Endopeptidase(MME)(AA52-750)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
MembraneMetallo-Endopeptidase(MME)(AA52-750)(Active)Protein
详情介绍：
Characteristics rhCD10, with Gly-Pro at the N- terminus, has a calculated MW of 80 kDa. The predicted N-terminus is Tyr 52. The reducing (R) protein migrates as 90-100 kDa due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH. The specific activity is >2500 pmol/min/μg.
Background Cluster of differentiation 10 (CD10) is also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110- kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer's disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).
Molecular Weight 80 kDa
NCBI Accession NP_000893
UniProt P08473
Pathways RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 20 mM Tris, 100 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Membrane Metallo-Endopeptidase (MME) (AA 52-750) (Active) Protein (ABIN2181505) Human Neprilysin, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Pardossi-Piquard, Dunys, Yu, St George-Hyslop, Alves da Costa, Checler: "Neprilysin activity and expression are controlled by nicastrin." in: Journal of neurochemistry, Vol. 97, Issue 4, pp. 1052-6, 2006 (PubMed).

Goodman, Febbraio, Simantov, Zheng, Shen, Silverstein, Nanus: "Neprilysin inhibits angiogenesis via proteolysis of fibroblast growth factor-2." in: The Journal of biological chemistry, Vol. 281, Issue 44, pp. 33597-605, 2006 (PubMed).

Cohen, Bunn, Franklin, Magill-Solc, Hartmann, Helfrich, Gilman, Folkvord, Helm, Miller: "Neutral endopeptidase: variable expression in human lung, inactivation in lung cancer, and modulation of peptide-induced calcium flux." in: Cancer research, Vol. 56, Issue 4, pp. 831-9, 1996 (PubMed).