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  • 产品名称：ComplementFactorD(CFD)(AA26-253)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ComplementFactorD(CFD)(AA26-253)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.2 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE).
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >70 pmol/min/μg.
Background Complement factor D (CFD) is also known as Adipsin, C3 convertase activator, Properdin factor D (PFD), which contains one peptidase S1 domain and belongs to the peptidase S1 family. CFD / Adipsin cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. CFD / Adipsin is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency which predisposes to invasive meningococcal disease.
Molecular Weight 25.2 kDa
UniProt P00746
Pathways Complement System
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Complement Factor D (CFD) (AA 26-253) (Active) protein (His tag) (ABIN2180874) Human Complement Factor D, His Tag on SDS-PAGE under reducing (R) condition. The gel ...
Background publications Sprong, Roos, Weemaes, Neeleman, Geesing, Mollnes, van Deuren: "Deficient alternative complement pathway activation due to factor D deficiency by 2 novel mutations in the complement factor D gene in a family with meningococcal infections." in: Blood, Vol. 107, Issue 12, pp. 4865-70, 2006 (PubMed).

White, Gribble, Corr, Large: "Jumping from a general hospital." in: General hospital psychiatry, Vol. 17, Issue 3, pp. 208-15, 1995 (PubMed).

White, Damm, Hancock, Rosen, Lowell, Usher, Flier, Spiegelman: "Human adipsin is identical to complement factor D and is expressed at high levels in adipose tissue." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9210-3, 1992 (PubMed).

Johnson, Gagnon, Reid: "Amino acid sequence of human factor D of the complement system. Similarity in sequence between factor D and proteases of non-plasma origin." in: FEBS letters, Vol. 166, Issue 2, pp. 347-51, 1984 (PubMed).