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  • 产品名称：FcFragmentofIgG,HighAffinityIa,Receptor(CD64)(FCGR1A)(AA16-288)(Active)protein(

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  • 产品厂商：ACROBiosystems
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简单介绍：
FcFragmentofIgG,HighAffinityIa,Receptor(CD64)(FCGR1A)(AA16-288)(Active)protein(Histag)
详情介绍：
Brand MABSol®
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 32.5 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human IgG1 in the SPR assay (Biacore 2000) with the KD < 5 nM.

SDS-PAGE: due to glycosylation.
Background Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
Molecular Weight 32.5 kDa
UniProt P12314
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-288) (Active) protein (His tag) (ABIN2181076) Human Fc gamma RI, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
image for Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-288) (Active) protein (His tag) (ABIN2181076) Immobilized Anti-CD64 MAb, Human IgG1 at 5μg/mL (100 μL/well) can bind Human CD64, Hi...
Background publications van Baal, Kenemans, Stehouwer, Peters-Muller, van Vugt, van der Mooren: "Sequentially combined hormone replacement therapy reduces impedance to flow within the uterine and central retinal arteries in healthy postmenopausal women." in: American journal of obstetrics and gynecology, Vol. 181, Issue 6, pp. 1365-73, 2000 (PubMed).

van Vugt, Kleijmeer, Keler, Zeelenberg, van Dijk, Leusen, Geuze, van de Winkel: "The FcgammaRIa (CD64) ligand binding chain triggers major histocompatibility complex class II antigen presentation independently of its associated FcR gamma-chain." in: Blood, Vol. 94, Issue 2, pp. 808-17, 1999 (PubMed).

Ernst, Duchemin, Miller, Anderson: "Molecular characterization of six variant Fcgamma receptor class I (CD64) transcripts." in: Molecular immunology, Vol. 35, Issue 14-15, pp. 943-54, 1999 (PubMed).

Edberg, Yee, Rakshit, Chang, Gokhale, Indik, Schreiber, Kimberly: "The cytoplasmic domain of human FcgammaRIa alters the functional properties of the FcgammaRI.gamma-chain receptor complex." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30328-33, 1999 (PubMed).

van Vugt, Heijnen, Capel, Park, Ra, Saito, Verbeek, van de Winkel: "FcR gamma-chain is essential for both surface expression and function of human Fc gamma RI (CD64) in vivo." in: Blood, Vol. 87, Issue 9, pp. 3593-9, 1996 (PubMed).