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  • 产品名称：ProgrammedCellDeath1Ligand2(PDCD1LG2)(AA20-219)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProgrammedCellDeath1Ligand2(PDCD1LG2)(AA20-219)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh B7-DC / PD-L2 / CD273 Fc Chimera at 1 μg/mL (100 μL/well) can bind rhPDCD-1 with a linear range of 7.5 - 500 ng/mL.

SDS-PAGE: due to glycosylation.
Background Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Molecular Weight 49.2 kDa
UniProt Q9BQ51
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 20-219) (Active) protein (Fc Tag) (ABIN2749238) Human PD-L2, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel...
Binding Studies (Bind) image for Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 20-219) (Active) protein (Fc Tag) (ABIN2749238) Immobilized Human PD-1, His Tag with a linear range of 0.16-2.5 μg/mL.
Binding Studies (Bind) image for Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 20-219) (Active) protein (Fc Tag) (ABIN2749238) Immobilized Human PD-1, Strep Tag with a linear range of 0.4-3 ng/mL.
Background publications Zhang, Henzel: "Signal peptide prediction based on analysis of experimentally verified cleavage sites." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 10, pp. 2819-24, 2004 (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Latchman, Wood, Chernova, Chaudhary, Borde, Chernova, Iwai, Long, Brown, Nunes, Greenfield, Bourque, Boussiotis, Carter, Carreno, Malenkovich, Nishimura, Okazaki, Honjo, Sharpe, Freeman: "PD-L2 is a second ligand for PD-1 and inhibits T cell activation." in: Nature immunology, Vol. 2, Issue 3, pp. 261-8, 2001 (PubMed).