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  • 产品名称：FcFragmentofIgG,LowAffinityIIIb,Receptor(CD16b)(FCGR3B)(AA17-200)(Active)protei

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  • 产品厂商：ACROBiosystems
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简单介绍：
FcFragmentofIgG,LowAffinityIIIb,Receptor(CD16b)(FCGR3B)(AA17-200)(Active)protein(Histag)
详情介绍：
Brand MABSol®
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.7 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human IgG with an estimated KD < 150 nM.
Background CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Molecular Weight 21.7 kDa
UniProt O75015
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B) (AA 17-200) (Active) protein (His tag) (ABIN2180704) Human Fc gamma RIIIB/CD16b, His Tag on SDS-PAGE under reducing (R) condition. The gel...
Background publications Bux, Stein, Bierling, Fromont, Clay, Stroncek, Santoso: "Characterization of a new alloantigen (SH) on the human neutrophil Fc gamma receptor IIIb." in: Blood, Vol. 89, Issue 3, pp. 1027-34, 1997 (PubMed).

Gessner, Radeke, Uciechowski, Resch, Schmidt: "[Activation-dependent expression of Fc gamma-receptors on glomerular mesangial cells]." in: Immunität und Infektion, Vol. 23, Issue 2, pp. 67-9, 1995 (PubMed).

Gessner, Grussenmeyer, Kolanus, Schmidt: "The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions." in: The Journal of biological chemistry, Vol. 270, Issue 3, pp. 1350-61, 1995 (PubMed).

Ravetch, Perussia: "Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions." in: The Journal of experimental medicine, Vol. 170, Issue 2, pp. 481-97, 1989 (PubMed).