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  • 产品名称：SemaDomain,ImmunoglobulinDomain(Ig),TransmembraneDomain(TM)andShortCytoplasmicD

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  • 产品厂商：ACROBiosystems
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简单介绍：
SemaDomain,ImmunoglobulinDomain(Ig),TransmembraneDomain(TM)andShortCytoplasmicDomain,(Semaphorin)4A(Sema4a)(AA33-683)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 72.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.
Background Semaphorin-4A (SEMA4A) is also known as Semaphorin-B (SEMAB), which belongs to the semaphorin family. SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one sema domain. SEMA4A is cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. SEMA4A plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. SEMA4A also promotes phosphorylation of TIMD2 and inhibits angiogenesis. SEMA4A promotes axon growth cone collapse and inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Molecular Weight 72.7 kDa
UniProt Q9H3S1
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4A (Sema4a) (AA 33-683) (Active) protein (His tag) (ABIN2181737) Human Semaphorin 4A, His Tag on SDS-PAGE under reducing (R) condition. The gel was st...
Background publications Abid, Ismail, Mehdi, Khaliq: "Identification of novel mutations in the SEMA4A gene associated with retinal degenerative diseases." in: Journal of medical genetics, Vol. 43, Issue 4, pp. 378-81, 2006 (PubMed).